10-06-2017, 07:09 AM
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Submitted by:Shinoy Ali
Supervised by
r. Maheep BhatnagarDepartment of Zoology
Mohanlal Sukhadiya University ,Udaipur
NITRIC OXIDE
Neurotransmitter which is a labile, free radical gas.
Not stored in Synaptic vesicles.
Not react with Receptor but diffuses into adjacent cells.
Act as Vasodilator.
Excessive NO is neurotoxic
SYNTHESIS
NADPH-d HISTOCHEMISTRY
As NO is a gas with half-life of 5 s, its direct measurement and localisation in the tissue is difficult.
Marker for nitric oxide synthase.
Used for the visualization of NOS-containing neuron.
The diaphorases are the class of enzyme catalyzes the reduction of NADPH to NADP,
NADPH + Tetrazolium NADP + Formazan
(Blue colour)
The technique provides a distinct image of particular neurons, dendritic trees and axonal networks