08-17-2017, 12:07 AM
Effect of Different Organic Amendments Addition into Azospirillum Bioinoculant with Lignite as Carrier Material
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INTRODUCTION
shelf life of microbial inoculant of Azospirillum lipoferum
Biofertilizers have come to stay in Indian dust, Paddy straw powder, Wood charcoal, Farmyard
agriculture since last three decades in view of their cost manure and Poultry manure with lignite as carrier material.
effectiveness, contribution to crop productivity, soil
sustainability and ecofriendly characters. Biofertilizers
MATERIALS AND METHODS
form an integral part of Integrated Plant Nutrient Supply
system (IPNS) and organic farming which constitutes
Production of Carrier Based Inoculants
the present as well as future mandate of Indian Preparation of Broth: Nitrogen Free malic acid broth was
agriculture. Biofertilizers manufactured in India presently prepared as per the composition and inoculated with
are carrier based, in general and suffer from short shelf Azospirillum lipoferum in 250 ml conical flask. It was
life, poor quality, high contamination and low and allowed to multiply by incubating at 30 2 C in a
unpredictable field performances. Death of the psychrotherm. (Model environ shaker 3597-1L BGM)
organisms in the inoculated seeds is one of the incubator cum shaker at 100 rpm for 72 hours. The broth
important factors contributing the failure of inoculation containing approximately 16.0 x 10 /ml was used for mixing
response in field condition. Research conducted on the with carriers.