(Sodium dodecyl Sulphate Polyacrylamide Gel Electrophoresis)

[susheelkumar]

[attachment=6127]

---

Presented By
Dr.A.G.Ingale
Associate professor
Department Of Biotechnology

(Sodium dodecyl Sulphate Polyacrylamide Gel Electrophoresis)

What is SDS-PAGE?

SDS (Sodium dodecyl Sulphate) is a detergent used to denature proteins and give them a negative charge
PAGE: Polyacrylamide Gel Electrophoresis

It is a technique to separate proteins by their molecular weight

What is in the Sample Buffer?
Tris buffer to provide appropriate pH
SDS (sodium dodecyl sulphate) detergent to dissolve proteins and give them a negative charge
Glycerol to make samples sink into wells
Bromophenol Blue dye to visualize samples
Why Use Acrylamide Gels to Separate Proteins?


Acrylamide gel: tight matrix
Ideal for protein separation
Smaller pore size than agarose
Proteins much smaller than intact
chromosonal DNA
average amino acid =
110 Dalton

Why do we need to denature the proteins?

For more information about this article,please follow the link:
http://davidson.edu/academic/biology/cou...SPAGE.html