This article is presented by:
Ji Yoon Park
Dept. of Biochem
Hanyang University


Abstract

1. Succeeded in solving an instance of a 6-variable 11-
clause 3-SAT problem on a gel-based DNA computer

2. Separation were performed using probes covalently
bound to polyacrylamide gel

3. During the entire computation, DNA was retained
within a single gel and moved via electrophoresis

4. To be readily automatable and should be suitable for
problems of a sign ....etc

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Gas Chromatography
A Quick Historical Perspective
1903 Adsorption chromatography
1952 Gas chromatography (GLC)
1968 High performance liquid chromatography (HPLC)


Gas Chromatography
HPLC
Mobile phase changes
Constant temperature
Compounds partition from the mobile phase based on solubility.
Elution is generally time or volume dependent


GC
Mobile phase is constant
Increasing temperature
Compounds partition from the mobile phase based on volatility.
Elution ....etc

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1 Introduction
Chromatography is a powerful separation method that finds applications in all branch of science. Chromatography was invented and named by the Russian botanist Mikhail Tswett shortly after the turn of the last century. He employed this technique to separate various plant pigments such as chlorophylls and xanthophylls by passing solutions of these compounds through a glass column packed with finely divided calcium carbonate. The separated species appeared as colored bands on the column, which accounts fo ....etc

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Presented By
Dr.A.G.Ingale
Associate professor
Department Of Biotechnology
(Sodium dodecyl Sulphate Polyacrylamide Gel Electrophoresis)


What is SDS-PAGE?

SDS (Sodium dodecyl Sulphate) is a detergent used to denature proteins and give them a negative charge
PAGE: Polyacrylamide Gel Electrophoresis

It is a technique to separate proteins by their molecular weight

What is in the Sample Buffer?
Tris buffer to provid ....etc

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please send me the ppt of instrumentation of gas chromatography i need it .. till tomorrow morning till 8a.m. ....etc

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Abstract
Both the rolling thin film oven test (RTFOT) and the short-term oven aging (STOA) methods are used in the laboratory to represent
the aging of an asphalt binder during plant mixing, transportation and paving. The RTFOT is conducted at 163 C for 85 min, and the
recommended STOA methods are to heat the loose mix in a forced draft oven either at 135 C for 4 h or at 154 C for 2 h dependent on
the asphalt binder s stiffness. The actual time of short-term aging in the field varies depending on hauling dista ....etc

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I have t present a seminar on gel filtration chromatography.Need some good and useful ppt's ....etc

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